ECOLOGY AND BEHAVIOR Aromatherapy in the Mediterranean Fruit Fly (Diptera: Tephritidae): Sterile Males Exposed to Ginger Root Oil in Prerelease Storage Boxes Display Increased Mating Competitiveness in Field-Cage Trials

Previous research showed that exposure to ginger root, Zingiber officinale Roscoe, oil increased the mating success of mass-reared, sterile males of the Mediterranean fruit ßy, Ceratitis capitata (Wiedemann). This work, however, involved the exposure of small groups of males (n 25) in small containers (volume 400 ml). Several sterile male release programs use plastic adult rearing containers (so-called PARC boxes; hereafter termed storage boxes; 0.48 by 0.60 by 0.33 m) to hold mature pupae and newly emerged adults before release ( 36,000 ßies per box). The objective of the current study was to determine whether the application of ginger root oil to individual storage boxes increases the mating competitiveness of sterile C. capitata males. Irradiated pupae were placed in storage boxes 2 d before adult emergence, and in the initial experiment (adult exposure) ginger root oil was applied 5 d later (i.e., 3 d after peak adult emergence) for 24 h at doses of 0.0625, 0.25, 0.5, 1.0, and 2.0ml. In a secondexperiment (pupal-adult exposure), ginger root oilwas applied to storageboxes immediately after pupal placement and left for 6 d (i.e., 4 d after peak adult emergence) at doses of 0.25and1.0ml.UsingÞeldcages,weconductedmating trials inwhichginger rootoil-exposed(treated) or nonexposed (control) sterilemales competed against wild-likemales for copulations withwild-like females. After adult exposure, treatedmales had signiÞcantly highermating success than controlmales for all doses of ginger root oil, except 2.0 ml. After pupal-adult exposure, treated males had a signiÞcantly highermating success than control males for the 1.0-ml but not the 0.25-ml dose of ginger root oil. The results suggest that ginger root oil can be used in conjunction with prerelease, storage boxes to increase the effectiveness of sterile insect release programs.